Image Analysis System for Quantitative Immunofluorescence Measurement

Magazine
  • 2002
  • September
  • Page 19

Yuri M. Kolesnik, Maxim A. Orlovsky

Dept of Pathophysiology, Zhaporozhye State Medical University, Ukraine

Monday, June 10, 2013 - 10:30

Immunofluorescence methods for hormone-containing cells exposure are widely used but the quantitative nature of the immunostaining is frequently ignored. In this study, quantitative measurements of fluorescent pancreatic beta-cells before and after glucose stimulation were used to determine parameters characterizing insulin synthesis and secretory activity. Beta-cells in Wistar rats pancreatic islets were stained using indirect immunohistochemistry, with subsequent processing on image analysis systems. The presence of three independent beta-cell classes with different insulin synthesis and secretion activity was shown by subsequent cluster analysis and confirmed by changes in insulin blood level.

Search for back issues: 

From the list below

select year followed by issue

Search for a back issue

from our archives

Website developed by S8080 Digital Media