Light sheet microscopy boosts live cell imaging
Image: Hong Kong University of Science and Technology researchers showcase LiTone Line Bessel Sheet microscopy [HKUST].
In a breakthrough for cell biology, researchers at The Hong Kong University of Science and Technology (HKUST) have developed a microscope to capture 3D live cell videos at a resolution to at least rival confocal microscopy.
While an existing confocal microscope can capture 3D bio-images, intense light exposure from the laser disrupts cell activities limiting the study of cells.
Given this, Professor Du Shengwang from Physics and the Super-Resolution Imaging Center and Professor Michael Loy from Physics at HKUST, and colleagues, developed the LiTone Line Bessel Sheet microscope, which uses ultrathin - non-diffracting - Bessel light sheets to image samples.
According to the researchers, the instrument is 1000 times less photo-toxic and 1000 times faster than a typical confocal model microscope, allowing much higher temporal resolution.
"It is a powerful, sophisticated technology, but we make it simple to users so that it can be operated by biologists with minimal amount of training," highlights Shengwang.
"For the first time, scientists can study cells in much greater details," he adds. That could eventually help scientists unlock the mystery of how certain diseases were formed and developed in a cellular level."
Research published in Nature Scientific Reports.